{"id":7889,"date":"2023-08-11T18:45:36","date_gmt":"2023-08-11T18:45:36","guid":{"rendered":"http:\/\/salishseasciences.org\/blog\/?p=7889"},"modified":"2023-08-11T18:45:38","modified_gmt":"2023-08-11T18:45:38","slug":"august-10th-coffee-finishing-pcr-and-sunset","status":"publish","type":"post","link":"https:\/\/salishseasciences.org\/blog\/august-10th-coffee-finishing-pcr-and-sunset\/","title":{"rendered":"August 10th: Coffee, Finishing PCR, and Sunset!"},"content":{"rendered":"\n<p>Hey Blog!&nbsp;<\/p>\n\n\n\n<p>Welcome back to the S(l)a(y)lish Sea Sciences blog written by the amazing group of two: Una and Millie! This morning, a few of us stopped in town for coffee. We went to The Crow\u2019s Nest! I ordered a pistachio rose latte, which I thought would be more floral-y and nutty but it wasn\u2019t, and ended up just tasting like a latte. Props to the barista: she worked like a pro, making 4 different drinks all at once. <\/p>\n\n\n\n<figure class=\"wp-block-image is-resized\"><img loading=\"lazy\" src=\"https:\/\/lh4.googleusercontent.com\/55fTKlWIrPBLnvoPeJqEsomHN0cJTLagdOIknSRq-PQEU6hxXwV6p77pQ2cGk4L-Le0Gvzj8rvRnR546UalvInvtfmshxvAN_0M2WrPR83Ar5F6pyoy3wqrw49fBzFY2c6CZD3y9KowTswVpn9pJvus\" alt=\"\" width=\"614\" height=\"818\"\/><figcaption>Crow&#8217;s Nest barista barista-ing. Photo creds to Millie!<\/figcaption><\/figure>\n\n\n\n<p>After working on our projects for 30 minutes, we went to a local high school to finish up what we did yesterday: PCR for the Invasive Mussel Project! After working carefully to measure out PCR cocktails with micropipettes, letting our PCR tubes run through the thermocycler, and setting up our gel electrophoresis, we waited excitedly to see the results. We expected some cute glowing lines demonstrating gene sequence length that would allow us to determine the mussels\u2019 species when compared to the DNA marker. Instead, we got nothing at all! Other than the important life lesson that science can be frustrating and disappointing, I guess. Sam Garson, who was directing us, guessed that the issue was something that unfortunately we do not remember.<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img src=\"https:\/\/lh6.googleusercontent.com\/OeIrG8y1BAXtmc85mOLJJdKxnBVonIAMa8y759aZ81ZRkRHMjW8lXcbSJZtCKsz5-Co1LYiX-WgbFrWGauz7JMdeMVmbIlQ-Vn0CVSYO89jsXH6zHJZHptYgbdC5pZtc5FDnyNtUVxRrrbNjiUzkKPk\" alt=\"\"\/><figcaption>Salish Sea Scientists are pros with micropipettes! Photo creds to Maya.<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><img src=\"https:\/\/lh4.googleusercontent.com\/7dPnmxgMTGvlEiOjkJtemIgUwnBD3Cr9PETMp_pRTx0SAO-vdJc0FSKILXtoKUq8nymJ9hpb0rmRtdzhuaaTObpvBDI1XozmzMZSbGyQo9zmKkPrO75vbFd2fVuUI2qAuN3tzs1qJzBT8m2Tn-ZxSGY\" alt=\"\"\/><figcaption>Millie practicing scalpel precision in only the most scientific way.<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><img src=\"https:\/\/lh4.googleusercontent.com\/Uu3C4Y5WutsVPHqk8FBsQ-Q4O5aoLLsLPb7E13iala2BZSnO65KddnoxEzS_-IITSnY7Zj_DyFCi2edu3OPsW_uLlYdvfEwDqzsNIfaBjZjEEYrLq2A8DBYCRLC2mlcHYiX4JNCpBbeV-CLDUK2zmKE\" alt=\"\"\/><figcaption>Cleo and Una in the lab, carefully investigating&#8230;something. Not sure what. Photo creds to Maya.<\/figcaption><\/figure>\n\n\n\n<p>Our guest speakers, Sofia Denkovski and Erin Casellas from Soundwatch, came in at 3 o\u2019clock and talked about Orcas! Specifically near the Salish Seas. They first talked about the different marine mammals in the Salish Seas, then went on to the scientific names of different whales, then talked about Orca identification. We did a fun game of Orca ID, identifying different orcas based on their saddle patches and dorsal fins. We learned that each saddle patch on an orca is like a fingerprint to a human\u2013none of them share the same one.<\/p>\n\n\n\n<p>After the informative presentation, Adam drove all of us down to South Beach. We watched the sunset, ate pie (the rhubarb pie was so good omg??), and climbed the cliffs and logs all while the wind blew in our faces, making us chilly. We took a lot of photos and watched the sky turn indigo. It was the perfect time to reminisce about our trip and all the memories we made, since this was our second to last day.<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img src=\"https:\/\/lh6.googleusercontent.com\/7_UUyLx4_vmfAxYsrnJx1mCBd2Epn5NEwY0ZCaLZef4MQjF4nNwpljFFIDw8MK0Hv3_Ky01tLspP-pV9OW-9Pj8cfv9XPDOFLBMOizslAnDTncP4T-KM2vfUC1iWS7S3_bHzCFBXYW2eg1F8XdD2Mxg\" alt=\"\"\/><figcaption>Rhubarb pie is the best pie.<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><img src=\"https:\/\/lh6.googleusercontent.com\/dj72e1QdcLrFBNeoSDp_y8e_tWsoIyykpkj4J7NvmQGXKcZMs9W7mq5wNJHaOuMRhlo_B3Ir9u-NRJdFGcbFk_szfyY9EuujRs_I0bOBOyY5xextrrmm--XdQFGhtiWJkgb_oGFST9yQrpV_1QMVIbA\" alt=\"\"\/><figcaption>Riya doing &#8221; \ud83d\ude1b &#8220;.<\/figcaption><\/figure>\n\n\n\n<p>See you all tomorrow! I can&#8217;t believe this is almost over, time flies!<\/p>\n\n\n\n<p><img loading=\"lazy\" src=\"https:\/\/lh3.googleusercontent.com\/SrByct7u7SbLqECXrewZBx0y5x5S335P3hb-mJcGDJkBPof2rIDVtLgiEAGXJofVeBrm3-H63x6M7Q63K8UhF_HoxaDAX4_G6JUJRbEaioMgRrePGBpcJgg9f6892LnY8NsIVTNMfGW5SwRBnqV5Fcs\" width=\"624\" height=\"832\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Hey Blog!&nbsp; Welcome back to the S(l)a(y)lish Sea Sciences blog written by the amazing group of two: Una and Millie! This morning, a few of us stopped in town for coffee. We went to The Crow\u2019s Nest! I ordered a pistachio rose latte, which I thought would be more floral-y and nutty but it wasn\u2019t, and ended up just tasting like a latte. Props to the barista: she worked like a pro, making 4 different drinks all at once. After working on our projects for 30 minutes, we went to a local high school to finish up what we did yesterday: PCR for the Invasive Mussel Project! After working carefully &hellip; <a href=\"https:\/\/salishseasciences.org\/blog\/august-10th-coffee-finishing-pcr-and-sunset\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":211,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_s2mail":"yes"},"categories":[10],"tags":[],"_links":{"self":[{"href":"https:\/\/salishseasciences.org\/blog\/wp-json\/wp\/v2\/posts\/7889"}],"collection":[{"href":"https:\/\/salishseasciences.org\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/salishseasciences.org\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/salishseasciences.org\/blog\/wp-json\/wp\/v2\/users\/211"}],"replies":[{"embeddable":true,"href":"https:\/\/salishseasciences.org\/blog\/wp-json\/wp\/v2\/comments?post=7889"}],"version-history":[{"count":1,"href":"https:\/\/salishseasciences.org\/blog\/wp-json\/wp\/v2\/posts\/7889\/revisions"}],"predecessor-version":[{"id":7890,"href":"https:\/\/salishseasciences.org\/blog\/wp-json\/wp\/v2\/posts\/7889\/revisions\/7890"}],"wp:attachment":[{"href":"https:\/\/salishseasciences.org\/blog\/wp-json\/wp\/v2\/media?parent=7889"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/salishseasciences.org\/blog\/wp-json\/wp\/v2\/categories?post=7889"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/salishseasciences.org\/blog\/wp-json\/wp\/v2\/tags?post=7889"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}